Multiplexed Detection of Infectious Diseases With Microfluidic Loop-Mediated Isothermal Amplification and a Smartphone
New tools are needed to enable rapid detection, identification, and reporting of infectious viral and microbial pathogens in a wide variety of point-of-care applications that impact human and animal health. We report the design, construction, and characterization of a platform for multiplexed analysis of disease-specific DNA sequences that utilizes a smartphone camera as the sensor in conjunction with a handheld instrument that interfaces the phone with a silicon-based microfluidic chip. Utilizing specific nucleic acid sequences for four equine respiratory pathogens as representative examples, we demonstrated the ability of the system to use a single 15-μL droplet of test sample to perform selective positive/negative determination of target sequences, including integrated experimental controls, in approximately 30 minutes. The system achieves detection limits comparable to those obtained by laboratory-based methods and instruments. © 2017 IEEE.
Sun, F.; Chen, W.; Yu, H.; Omob, A.; Brisbin, R.; Ganguli, A.; Vemuri, V.; Strzebonski, P.; Cui, G.; Allen, K.J.; Desai, S.A.; Lin, W.; Nash, D.M.; Hirschberg, D.L.; Brooks, I.; Bashir, R.; and Cunningham, B.T., "Multiplexed Detection of Infectious Diseases With Microfluidic Loop-Mediated Isothermal Amplification and a Smartphone" (2017). SIAS Faculty Publications. 902.