"Multiplexed Detection of Infectious Diseases With Microfluidic Loop-Me" by F. Sun, W. Chen et al.
 

Multiplexed Detection of Infectious Diseases With Microfluidic Loop-Mediated Isothermal Amplification and a Smartphone

Publication Date

2017

Document Type

Conference Proceeding

Abstract

New tools are needed to enable rapid detection, identification, and reporting of infectious viral and microbial pathogens in a wide variety of point-of-care applications that impact human and animal health. We report the design, construction, and characterization of a platform for multiplexed analysis of disease-specific DNA sequences that utilizes a smartphone camera as the sensor in conjunction with a handheld instrument that interfaces the phone with a silicon-based microfluidic chip. Utilizing specific nucleic acid sequences for four equine respiratory pathogens as representative examples, we demonstrated the ability of the system to use a single 15-μL droplet of test sample to perform selective positive/negative determination of target sequences, including integrated experimental controls, in approximately 30 minutes. The system achieves detection limits comparable to those obtained by laboratory-based methods and instruments. © 2017 IEEE.

Volume

2017-December

First Page

241

Last Page

244

DOI

10.1109/HIC.2017.8227629

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